Here's a paragraph about the molds that may help to infer the reason of the advantages during observation of mold colonies: 'Because the structural components of molds are very delicate, even simple handling with an inoculating loop may result in mechanical disruption of their components.The followi ng culture technique es used to to avoid this disruption. After culturing, molds spores are deposited in the surface of the agar and incubated in a moist chamber at room temperature. Direct microscopic observation is then possible without fear of disruption or damage to the anatomical components'. It may be more beneficial in this aspect: allowing a full and healthy growth of the mold, that is, with its complete structure, then to be able to proceed to look through the microscope maybe it form, type of spore,sporangia or mycelium. In short, ensure that the mold to grow properly. Maybe it's not what you exactly were looking for but it could help you! Second Year of Microbiology bachelor's degree (University Of Puerto Rico in Arecibo) Paragraph taken of Capuccino/Sherman Microbiology.

Best Answer: Serial dilution direct count: Advantages: Count all the cells living or dead. Results would be quickly obtained. Disadvantage: Can't differentiate between living and dead cells. Direct count errors. Cells could be difficult to observe without stains or phase-contrast microscopy.

A laboratory Manual.Eight Edition. The following are some advantages of an agar plate verses a slant tube: 1.

Surface area- An agar plate has a much larger surface area: a. Easier to isolate individual colonies using the streak-plate method. Evaluate the colony shape, margin and elevation.

Can grow a larger number of ce lls. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range. Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large.

Abstract Manual counting of bacterial colony forming units (CFUs) on agar plates is laborious and error-prone. We therefore implemented a colony counting system with a novel segmentation algorithm to discriminate bacterial colonies from blood and other agar plates. A colony counter hardware was designed and a novel segmentation algorithm was written in MATLAB.

In brief, pre-processing with Top-Hat-filtering to obtain a uniform background was followed by the segmentation step, during which the colony images were extracted from the blood agar and individual colonies were separated. A Bayes classifier was then applied to count the final number of bacterial colonies as some of the colonies could still be concatenated to form larger groups. To assess accuracy and performance of the colony counter, we tested automated colony counting of different agar plates with known CFU numbers of S. Pneumoniae, P. Aeruginosa and M. Catarrhalis and showed excellent performance. Introduction Microbiological research techniques often rely on accurate determination of colony forming units (CFUs).

A majority of modern researchers came to the conclusion that the ancestors of Kyrgyz tribes had their origin in the most ancient tribal unions of /, /,,. Also, there follow from the oldest notes about the Kyrgyz that the definite mention of Kyrgyz ethnonym originates from the 6th century. Though it is obviously impossible to directly identify the and Kyrgyz, a trace of their ethnogenetical connections is apparent in archaeology, history, language and ethnography.

Routinely, this is done by aliquoting a small amount of a liquid culture and plating out several serial dilutions onto culture plates (Petri dishes containing semisolid medium). After incubation in appropriate conditions for the microorganism of choice, the colonies are counted to determine the number of CFU.

This is done by manually counting of colonies on plates illuminated by transmitted light. The concentration of bacteria in the original culture can then be calculated based on the assumption that each colony has raised from one single bacterium (colony forming unit, CFU). This process is time-consuming, tedious and error prone. There is a tendency to analyse only high dilutions of the initial culture as these have fewer colonies to count. Unfortunately, in low count assays minor counting errors have significant effects on the calculated concentration in the primary liquid medium. On the other hand, accurate counting of plates with high numbers of CFUs is error prone since it requires a high level of attention by the performer. Therefore, often only parts of a plate are analyzed and used to estimate the whole plate count after extrapolation.

Furthermore high numbers of CFUs on a plate can lead to false redults due to overcrowding of bacteria. This study aimed to design an automated colony counter which reliably detects, bacterial counts and colony size on semisolid agar plates of 85 mm diameter. The system should be suitable for the study of important human pathogens, such as Streptococcus pneumoniae, Moraxella catarrhalis and Pseudomonas aeruginosa. These bacteria are grown on diverse agar plates including Columbia blood sheep agar (CSBA), chocolate agar and brain heart infusion (BHI) agar plates. The system should also be user-friendly and cost-effective with an algorithm that is adaptable to other culture media and microorganisms. Bacterial strains and growth conditions A total of 7 clinical isolates of S.